Pii: S0165-0270(98)00127-7
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چکیده
A rapid fluorescence imaging system was developed and utilised to investigate the time-course of intracellular calcium concentration ([Ca]i) gradients generated by action potentials in CA1-CA3 pyramidal cells within brain slices of the rat hippocampus. The system, which is based on a fast commercial CCD camera, can acquire hundreds of 128×128 pixel images in sequence, with minimal inter-frame interval of 2.5 ms (400 frames/s) and 12 bit/pixel accuracy. By synchronising patch clamp recordings with image capture, the timing of transmembrane potential variation, ionic Ca current and Ca diffusion were resolved at the limit of the relaxation time for the dye–Ca binding reaction (approximately 5 ms at room temperature). Numerical simulations were used to relate measured fluorescence transients to the spatio-temporal distribution of intracellular Ca gradients. The results obtained indicate that dye reaction–diffusion contributes critically to shaping intracellular ion gradients. © 1999 Elsevier Science B.V. All rights reserved.
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تاریخ انتشار 1999